Usability application of multiplex polymerase chain reaction in the diagnosis of microorganisms isolated from urine of patients treated in cancer hospital

Authors

  • Zefiryn Cybulski Department of Microbiology, Greater Poland Cancer Centre, Poznań, Poland
  • Katarzyna Schmidt Genetic Medicine Laboratory CBDNA Research Centre, Poznań, Poland
  • Alicja Grabiec Department of Microbiology, Greater Poland Cancer Centre, Poznań, Poland
  • Zofia Talaga Department of Microbiology, Greater Poland Cancer Centre, Poznań, Poland
  • Piotr Bociąg Genetic Medicine Laboratory CBDNA Research Centre, Poznań, Poland
  • Jacek Wojciechowicz Genetic Medicine Laboratory CBDNA Research Centre, Poznań, Poland
  • Andrzej Roszak Department of Radiotherapy and Gynaecologic Oncology, Greater Poland Cancer Centre, Poznań, Poland, Department of Electroradiology, University of Medical Sciences Poznań, Poland
  • Witold Kycler Department of Oncological Surgery II, Greater Poland Cancer Centre, Poznań, Poland

Abstract

Background

The objective of this study was: i) to compare the results of urine culture with polymerase chain reaction (PCR) -based detection of microorganisms using two commercially available kits, ii) to assess antimicrobial susceptibility of urine isolates from cancer patients to chosen antimicrobial drugs and, if necessary, to update the recommendation of empirical therapy.

Materials and methods

A one-year hospital-based prospective study has been conducted in Greater Poland Cancer Centre and Genetic Medicine Laboratory CBDNA Research Centre in 2011. Urine cultures and urine PCR assay from 72 patients were examined

Results

Urine cultures and urine PCR assay from 72 patients were examined. Urine samples were positive for 128 strains from which 95 (74%) were identical in both tests. The most frequently isolated bacteria in both culture and PCR assay were coliform organisms and Enterococcus spp. The Gram negative bacilli were most resistant to cotrimoxazol. 77.2% of these bacilli and 100% of E. faecalis and S. agalactiae were sensitive to amoxicillin-clavulanic acid. 4.7% of Gram positive cocci were resistant to nitrofurantoin.

Conclusions

The PCR method quickly finds the causative agent of urinary tract infection (UTI) and because of this can help with making the choice of proper antimicrobial therapy at an early stage. It appears to be a viable alternative to the recommendations made in general treatment guidelines, in cases where diversified sensitivity patterns of microorganisms have been found.

Author Biographies

Zefiryn Cybulski, Department of Microbiology, Greater Poland Cancer Centre, Poznań, Poland

Katarzyna Schmidt, Genetic Medicine Laboratory CBDNA Research Centre, Poznań, Poland

Alicja Grabiec, Department of Microbiology, Greater Poland Cancer Centre, Poznań, Poland

Zofia Talaga, Department of Microbiology, Greater Poland Cancer Centre, Poznań, Poland

Piotr Bociąg, Genetic Medicine Laboratory CBDNA Research Centre, Poznań, Poland

Jacek Wojciechowicz, Genetic Medicine Laboratory CBDNA Research Centre, Poznań, Poland

Andrzej Roszak, Department of Radiotherapy and Gynaecologic Oncology, Greater Poland Cancer Centre, Poznań, Poland, Department of Electroradiology, University of Medical Sciences Poznań, Poland

Witold Kycler, Department of Oncological Surgery II, Greater Poland Cancer Centre, Poznań, Poland

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Published

2013-08-27

How to Cite

Cybulski, Z., Schmidt, K., Grabiec, A., Talaga, Z., Bociąg, P., Wojciechowicz, J., Roszak, A., & Kycler, W. (2013). Usability application of multiplex polymerase chain reaction in the diagnosis of microorganisms isolated from urine of patients treated in cancer hospital. Radiology and Oncology, 47(3). Retrieved from https://www.radioloncol.com/index.php/ro/article/view/1522

Issue

Section

Clinical oncology