Inhibition of cathepsin X enzyme influences the immune response of THP-1 cells and dendritic cells infected with Helicobacter pylori

Authors

  • Miha Skvarč Faculty of Medicine, Institute of Microbiology and Immunology
  • David Štubljar Faculty of Medicine, Institute of Microbiology and Immunology
  • Andreja Nataša Kopitar Faculty of Medicine, Institute of Microbiology and Immunology
  • Samo Jeverica Faculty of Medicine, Institute of Microbiology and Immunology
  • Bojan Tepeš AM Diagnostic Center, Rogaška slatina
  • Janko Kos Faculty of Pharmacy
  • Alojz Ihan Faculty of Medicine, Institute of Microbiology and Immunology

Abstract

Background. The immune response to Helicobacter pylori importantly determines the outcome of infection as well as the success of eradication therapy. We demonstrate the role of a cysteine protease cathepsin X in the immune response to H. pylori infection.
Materials and methods. We analysed how the inhibition of cathepsin X influenced the immune response in experiments when THP-1 cells or dendritic cells isolated from patients were stimulated with 48 strains of H. pylori isolated from gastric biopsy samples of patients which had problems with the eradication of bacteria.
Results. The experiments, performed with the help of a flow cytometer, showed that the expression of Toll-like receptors (TLRs), especially TLR-4 molecules, on the membranes of THP-1 cells or dendritic cells was higher when we stimulated cells with H. pylori together with inhibitor of cathepsin X 2F12 compared to THP-1 cells or dendritic cells stimulated with H. pylori only, and also in comparison with negative control samples. We also demonstrated that when we inhibited the action of cathepsin X in THP-1 cells, the concentrations of pro-inflammatory cytokines were lower than when THP-1 cell were stimulated with H. pylori only.
Conclusions. We demonstrated that inhibition of cathepsin X influences the internalization of TLR-2 and TLR-4. TLR-2 and TLR-4 redistribution to intra-cytoplasmic compartments is hampered if cathepsin X is blocked. The beginning of a successful immune response against H. pylori in the case of cathepsin X inhibition is delayed.

Author Biographies

Miha Skvarč, Faculty of Medicine, Institute of Microbiology and Immunology

David Štubljar, Faculty of Medicine, Institute of Microbiology and Immunology

Andreja Nataša Kopitar, Faculty of Medicine, Institute of Microbiology and Immunology

Samo Jeverica, Faculty of Medicine, Institute of Microbiology and Immunology

Bojan Tepeš, AM Diagnostic Center, Rogaška slatina

Janko Kos, Faculty of Pharmacy

Alojz Ihan, Faculty of Medicine, Institute of Microbiology and Immunology

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Published

2013-08-27

How to Cite

Skvarč, M., Štubljar, D., Kopitar, A. N., Jeverica, S., Tepeš, B., Kos, J., & Ihan, A. (2013). Inhibition of cathepsin X enzyme influences the immune response of THP-1 cells and dendritic cells infected with Helicobacter pylori. Radiology and Oncology, 47(3). Retrieved from https://www.radioloncol.com/index.php/ro/article/view/1804

Issue

Section

Experimental oncology